Anti-Thyroid peroxidase/TPO Monoclonal Antibody for IF-P, IHC-P, ELISA - P07202

Core Product Specifications and Parameters

Biological Background: Thyroid Peroxidase/TPO Function and Localization

• Thyroid peroxidase (TPO), also known as MSA, TPX, or TDH2A, is a critical enzyme in thyroid hormone biosynthesis.
• Its primary function is the iodination and coupling of tyrosine residues in thyroglobulin to form the active thyroid hormones triiodothyronine (T3) and thyroxine (T4).
• TPO is a membrane-bound glycoprotein localized at the cell surface and within intracellular membranes of thyroid follicular cells.
• As a heme-containing peroxidase, it requires hydrogen peroxide as a substrate and binds calcium and iron for enzymatic activity.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for proper folding and stability.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional roles.
• Genetic variants in TPO are associated with congenital hypothyroidism, underscoring its essential role in thyroid function.
• The protein belongs to the thyroid peroxidase family and is a key autoantigen in autoimmune thyroid disorders (e.g., Hashimoto's thyroiditis).

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 872–933 near the C-terminus, facilitating detection of full-length TPO and possibly certain isoforms.
• For immunofluorescence (IF-P), optimized permeabilization and fixation are recommended to access the membrane-localized antigen.
• In immunohistochemistry (IHC-P), standard antigen retrieval methods (e.g., heat-induced epitope retrieval) may enhance staining of paraffin-embedded thyroid tissues.
• For ELISA applications, validate the antibody pair and assay conditions with appropriate positive and negative controls to ensure sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing platform for IVD raw materials, supporting workflow from concept to clinic. This high-quality anti-TPO monoclonal antibody is part of our extensive catalog of validated antibodies and ancillary reagents for thyroid function testing and autoimmune disease research. Beyond standard catalog products, we offer custom sourcing for rare targets, bulk supply, and technical consultation to accelerate your assay development and production.