Anti-Thyroglobulin Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P01266

Core Product Specifications and Parameters

Biological Background: Thyroglobulin Function and Localization

• Thyroglobulin (TG) is a large dimeric glycoprotein precursor essential for the synthesis of the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Related references: PMID:17532758, PMID:32025030
• Within the thyroid follicle lumen, selected tyrosine residues of TG undergo iodination followed by oxidative coupling to form T3 and T4. Related references: PMID:32025030
• Following re‑internalization into thyrocytes, lysosomal proteolysis of TG releases T3 and T4, which are then secreted into the bloodstream. Related references: PMID:32025030
• One TG dimer yields approximately seven thyroid hormone molecules. Related references: PMID:32025030
• TG is specifically expressed in the thyroid gland and is secreted into the follicular lumen.
• The protein features extensive glycosylation, iodination, and disulfide bond formation, and is annotated with keywords including glycoprotein, hormone, and iodination.
• Post‑translational modifications of TG include iodination of tyrosine residues, sulfation, and extensive N‑linked glycosylation, as reflected in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (amino acids 1000–1100) of human Thyroglobulin, suggesting potential cross‑reactivity with mouse and rat, as listed.
• For IF‑P, consider permeabilization conditions appropriate for secreted proteins; fixation with paraformaldehyde is recommended.
• For IHC‑P, antigen retrieval may be required; citrate buffer (pH 6.0) or EDTA (pH 9.0) protocols can be tested.
• In ELISA, the antibody can be used as capture or detection; validate pairing in the sample matrix.
• Although not validated for Western blot, if attempted, optimize transfer conditions for high‑molecular‑weight proteins (~305 kDa).
• Since thyroglobulin is highly abundant in thyroid tissue, dilution factors for IHC and ELISA may need adjustment to avoid saturation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one‑stop access to validated IVD raw materials, including rabbit monoclonal antibodies like this anti‑Thyroglobulin clone. Our portfolio offers matched antibody pairs, bulk reagents, and customized sourcing for thyroid biomarker development. Let CamelBio accelerate your assay development from concept to clinic with reliable supply chain support.