Anti-Thymidylate Synthase (TYSY) Polyclonal Antibody, KO Validated - P04818

Core Product Specifications and Parameters

Biological Background: Thymidylate Synthase (TYSY) Function and Localization

• Thymidylate synthase (TYMS, also known as TYSY, TS, TMS, HST422, SY) is a critical enzyme in the de novo synthesis of thymidylate (dTMP), an essential precursor for DNA replication and repair.
• Catalyzes the reductive methylation of 2′-deoxyuridine 5′-monophosphate (dUMP) to thymidine 5′-monophosphate (dTMP), using 5,10-methylenetetrahydrofolate as a one-carbon donor and reductant.
• Contributes to both mitochondrial and nuclear de novo thymidylate biosynthesis, supporting genome integrity in these compartments.
• Localizes to the nucleus, cytoplasm, and mitochondrion, including the mitochondrial matrix and inner membrane, reflecting its dual role in DNA synthesis.
• Subject to post-translational modifications, including phosphorylation and ubiquitination, which may regulate its activity and stability.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional roles.
• Disease-associated variants are linked to dyskeratosis congenita and other disorders, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 220–313) of human TYSY, which may confer specificity across species due to high conservation in this domain. Consider validating cross‑reactivity in your model system.
• For western blotting, the expected molecular weight is approximately 36 kDa. Both nuclear and cytoplasmic fractions can be examined; mitochondrial enrichment may also be considered.
• In immunohistochemistry (IHC) and immunofluorescence (IF/ICC), both nuclear and cytoplasmic staining patterns may be observed. Optimize antigen retrieval and use appropriate negative controls.
• When setting up ELISA or other quantitative assays, include recombinant TYSY protein or known positive lysates as standards to confirm sensitivity and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. This anti‑TYSY antibody supports research into nucleotide metabolism, cancer biology, and mitochondrial disorders, and CamelBio is ready to assist with your custom sourcing, technical consultation, and supply chain needs.