Anti-Thymidine Kinase 1 (TK1) Rabbit Monoclonal Antibody for WB and ELISA - P04183

Core Product Specifications and Parameters

Biological Background: Thymidine Kinase 1 Function and Localization

• Thymidine Kinase 1 (TK1) is a cell-cycle-regulated enzyme critical for nucleotide metabolism. Related references: PMID:9575153
• It catalyzes the conversion of thymidine into thymidine monophosphate, the first step in the salvage pathway of DNA synthesis. Related references: PMID:22385435
• TK1 expression is transcriptionally restricted to the S phase of the cell cycle, coinciding with transient oscillations in intracellular dTTP concentration.
• The enzyme also phosphorylates nucleoside analog prodrugs, including anticancer and antiviral agents such as AraC and AZT. Related references: PMID:22385435
• Subcellular localization is predominantly cytoplasmic.
• The protein has a molecular weight of approximately 25.5 kDa and contains ATP-binding and metal-binding (zinc) domains, as well as phosphorylation and acetylation sites.
• TK1 belongs to the thymidine kinase family and is part of the reference proteome.

Experimental Guidance and Technical Tips

• For Western blot (WB), use standard reducing SDS-PAGE and transfer conditions. The target protein is predicted at approximately 25 kDa; consider loading a pre-stained protein ladder to confirm molecular weight. Validate antibody specificity in cell lines known to express TK1, ideally after synchronization to enrich S-phase cells.
• For ELISA, the immunogen sequence (amino acids 1–100) may be used to coat plates for direct ELISA. Alternatively, a capture format using anti-TK1 as detection can be developed; optimize coating and blocking conditions.
• Due to the cell-cycle-dependent expression, signal intensity may vary; incorporate appropriate controls (e.g., G1 vs. S phase lysates) to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies IVD manufacturers and research laboratories with validated antibodies and raw materials for biomarker detection and disease research. This anti-Thymidine Kinase 1 product supports studies in cell proliferation, cancer diagnostics, and nucleotide metabolism. As a comprehensive sourcing partner, CamelBio offers one-stop access to antibody pairs, bulk reagents, and technical consultation—helping you bridge the gap from concept to clinical application.