Anti-Thymidine Kinase 1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04183

Core Product Specifications and Parameters

Biological Background: Thymidine Kinase 1 Function and Localization

• Thymidine kinase 1 (TK1), encoded by the TK1 gene, is a cytosolic enzyme in the nucleotide salvage pathway that converts thymidine into thymidine monophosphate (PubMed:9575153, PubMed:22385435). Related references: PMID:9575153, PMID:22385435
• TK1 expression is cell-cycle-regulated and restricted to the S phase, coinciding with fluctuations in intracellular dTTP concentrations.
• The enzyme plays a critical role in activating anticancer and antiviral nucleoside analog prodrugs, such as cytarabine (AraC) and zidovudine (AZT) (PubMed:22385435). Related references: PMID:22385435
• Subcellularly, TK1 is localized in the cytoplasm, where it functions in DNA precursor synthesis.
• Structural and functional features include ATP and zinc binding, and regulation via phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 15-194) covers a substantial N-terminal to central region, likely yielding broad epitope recognition; consider this when interpreting cross-reactivity data.
• For Western blot, the predicted molecular weight is ~25 kDa; validate band specificity using appropriate positive (e.g., proliferating cell lines) and negative controls.
• In IHC-P, antigen retrieval conditions may require optimization; TK1 expression is highest in S-phase cells, so choose tissues with high proliferative index.
• For IF/ICC, confirm colocalization with cytoplasmic markers under consistent fixation and permeabilization protocols.
• ELISA performance may vary with coating conditions; pair with a validated capture/detection system for best results.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides a streamlined sourcing solution for high-quality IVD antibodies like this anti-Thymidine Kinase 1 polyclonal antibody, supporting nucleotide metabolism and cancer biomarker research.
• We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialize in rare-target raw-material procurement to accelerate your assay development.
• From concept to clinic, our one-stop model offers technical services and consulting, ensuring reliable access to critical raw materials for diagnostic manufacturers, labs, and research institutes.