Anti-THRSP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92748

Core Product Specifications and Parameters

Biological Background: THRSP Function and Localization

• THRSP, also known as Spot 14 (S14), is a thyroid hormone-inducible hepatic protein encoded by the THRSP gene. It is a small protein of approximately 17 kDa.
• It plays a critical role in the regulation of lipogenesis, particularly in the lactating mammary gland, and is important for the biosynthesis of medium-chain fatty acid triglycerides.
• THRSP may modulate lipogenesis by interacting with MID1IP1, thereby preventing MID1IP1 from interacting with ACACA (acetyl-CoA carboxylase alpha) (By similarity).
• It is thought to function as a transcriptional coactivator, potentially modulating the transcription factor activity of thyroid hormone receptor beta (THRB).
• Subcellularly, THRSP is localized to both the nucleus and the cytoplasm, consistent with its roles in transcription and lipid metabolism.
• Tissue expression is predominantly in tissues active in triglyceride synthesis, highlighting its metabolic specificity.
• Key biological processes associated with THRSP include lipid biosynthesis, lipid metabolism, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length sequence (aa 1-146); this polyclonal antibody may recognize multiple epitopes, potentially providing robust detection in denaturing Western blot conditions.
• For WB, ensure adequate resolution for the 17 kDa protein by using high-percentage gels and appropriate molecular weight markers. Consider a standard blocking buffer and optimize primary antibody dilution.
• In IF/ICC, validate the expected nucleocytoplasmic localization in a cell line known to express THRSP. Fixation and permeabilization conditions may affect the staining pattern.
• For ELISA, perform checkerboard titrations to determine the optimal antigen coating concentration and antibody dilution.
• The antibody cross-reacts with mouse and rat, as indicated; validate species-specific expression profiles in relevant tissues (e.g., liver, mammary gland).
• Upon arrival, the antibody is shipped on ice; store at -20°C in small aliquots to avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. For your lipid metabolism and transcription regulation studies, we provide access to validated THRSP antibodies and a full portfolio of ancillary reagents. Whether you need bulk monoclonal/polyclonal antibodies, matched antibody pairs, or rare target sourcing, CamelBio supports your journey from concept to clinic.