Anti-Thromboxane synthase Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24557

Core Product Specifications and Parameters

Biological Background: Thromboxane synthase Function and Localization

• Thromboxane synthase (TBXAS1), also known as cytochrome P450 5A1, is encoded by the TBXAS1 gene in humans. It is a key enzyme in the arachidonic acid cascade.
• Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent vasoconstrictor and inducer of platelet aggregation (PubMed:11097184, PubMed:11297515, PubMed:22735388).
  Related references: PMID:11097184 PMID:11297515 PMID:22735388
• Also cleaves PGH2 to produce 12-hydroxyheptadecatrienoic acid (12-HHT) and malondialdehyde, a DNA damage mediator, in stoichiometric amounts equivalent to TXA2 (PubMed:11297515, PubMed:22735388, PubMed:9873013).
  Related references: PMID:11297515 PMID:22735388 PMID:9873013
• Displays dehydratase activity toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE), converting it to 15-KETE and 15-HETE (PubMed:17459323).
  Related references: PMID:17459323
• Localized to the endoplasmic reticulum membrane, where it functions as a monooxygenase and oxidoreductase requiring heme and iron cofactors.
• Expressed in platelets, lung, kidney, spleen, macrophages, and lung fibroblasts, consistent with its role in hemostasis and inflammation.
• Keywords include alternative splicing and disease variant, reflecting its association with Ghosal hematodiaphyseal dysplasia (GHOSAL) and bleeding disorder platelet-type 14 (BDPLT14).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 355-534 of human thromboxane synthase; the antibody is expected to detect the full-length protein (~61 kDa) by Western blot.
• For WB, test in platelet lysates or thromboxane synthase-expressing cell lines; consider using a reducing agent and appropriate blocking buffer to minimize non-specific binding.
• For IHC-P, antigen retrieval may be necessary; validate in positive control tissues such as lung or kidney sections, and include isotype controls.
• In ELISA, the antibody can be used as a capture or detection reagent; optimization of coating buffer and concentration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering a comprehensive range of antibodies, recombinant proteins, and ancillary reagents for diagnostic development. Our thromboxane synthase antibody supports research in thrombosis, cardiovascular disease, and platelet biology, and we can supply validated antibody pairs, bulk reagents, and rare-target raw materials to accelerate your assay development. Contact us to discuss your specific requirements.