Anti-Thromboxane Synthase Rabbit Monoclonal Antibody for WB and ELISA - P24557

Core Product Specifications and Parameters

Biological Background: Thromboxane-A Synthase Function and Localization

• Thromboxane-A synthase (TBXAS1), also known as cytochrome P450 5A1 and hydroperoxy icosatetraenoate dehydratase, belongs to the cytochrome P450 superfamily and is primarily involved in eicosanoid metabolism.
• Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent vasoconstrictor and platelet aggregation agonist, and co-produces 12-hydroxyheptadecatrienoic acid (12-HHT) and malondialdehyde (MDA) in stoichiometric amounts. Related references: PMID:11097184 PMID:11297515 PMID:22735388 PMID:9873013
• Exhibits dehydratase activity, converting (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) to 15-KETE and 15-HETE. Related references: PMID:17459323
• Localized to the endoplasmic reticulum membrane, where it functions as a monooxygenase and isomerase, requiring heme and iron cofactors.
• Predominantly expressed in platelets, lung, kidney, spleen, macrophages, and lung fibroblasts, consistent with its roles in hemostasis and inflammation.
• Alternative splicing generates multiple isoforms; disease-associated variants are linked to Ghosal hematodiaphyseal dysplasia and bleeding disorder platelet-type 14 (BDPLT14).
• Annotated with keywords including Fatty acid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, and Reference proteome, reflecting its central role in lipid mediator production.

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic peptide spanning the N-terminal amino acids 1–100 of human Thromboxane synthase (P24557); this region may confer target specificity across isoforms and species.
• For Western blot (WB), the protein carries a predicted molecular weight of approximately 61 kDa; consider using platelet-rich plasma, lung tissue, or macrophage lysates as positive controls.
• ELISA applications may employ direct or capture formats; validate the antibody’s performance in the relevant sample matrix and with appropriate recombinant protein standards.
• Cross-reactivity with mouse is confirmed, making this antibody suitable for translational models; optimization of dilutions and detection conditions is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and technical services, spanning from early-stage research to clinical implementation. For thromboxane synthase, a key enzyme in prostaglandin metabolism and platelet function, we supply validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate your assay development. Our team also specializes in sourcing rare-target raw materials and providing expert consulting, ensuring you have a reliable partner for every step of your project.