Anti-THRAP3 Polyclonal Antibody for WB - Q9Y2W1

Core Product Specifications and Parameters

Biological Background: THRAP3 Function and Localization

• THRAP3, also known as TRAP150 and BCLAF2, is a thyroid hormone receptor-associated protein encoded by the THRAP3 gene.
• Involved in pre-mRNA splicing, where it remains associated with spliced mRNA through interactions with the exon junction complex (EJC) and can trigger mRNA decay independent of nonsense-mediated decay.
• Regulates mRNA stability, notably as a component of the SNARP complex that stabilizes cyclin D1 (CCND1) mRNA by binding its 3' end, and participates in nuclear mRNA decay.
• Functions in DNA damage response; is excluded from DNA damage sites in parallel with transcription inhibition, a process that may involve the SNARP complex.
• Although initially identified as part of the TRAP/Mediator complex, it is not a stable subunit but cooperates with HELZ2 to enhance PPARG-mediated transcriptional activation, playing a role in adipocyte differentiation.
• Acts as a coactivator of the circadian clock by promoting the transcriptional activity and target gene binding of the CLOCK-BMAL1 heterodimer. Related references: PMID:24043798
• Subcellularly localized to the nucleus, including the nucleoplasm and nuclear speckles.
• Post-translationally modified by acetylation, phosphorylation, ADP-ribosylation, ubiquitination, and methylation, with ubiquitous tissue expression.

Experimental Guidance and Technical Tips

• The immunogen (aa 340-500) lies within an internal region of THRAP3, which may provide specificity across isoforms; validate antibody recognition in your target cell or tissue system.
• For Western blot (WB), a band around 109 kDa is expected; use appropriate loading controls and confirm in human, mouse, or rat samples based on reported cross-reactivity.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and primary antibody concentration; include positive and negative tissue controls.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays; consider using a monoclonal alternative if high reproducibility is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers, laboratories, and researchers with a comprehensive portfolio of IVD raw materials. This THRAP3 antibody supports investigations into pre-mRNA splicing, mRNA metabolism, and circadian biology. Contact us for validated antibody pairs, bulk reagents, and customized sourcing solutions tailored to your assay development—from concept through clinical application.