Anti-THPO Polyclonal Antibody for WB, ELISA - P40225

Core Product Specifications and Parameters

Biological Background: Thrombopoietin Function and Localization

• THPO (thrombopoietin), also known as ML, MGDF, MPLLG, and THCYT1, is a lineage-specific cytokine crucial for megakaryopoiesis.
• It specifically stimulates the proliferation and maturation of megakaryocytes from their committed progenitor cells, acting at a late stage of development.
• THPO is the primary physiological regulator of platelet production, maintaining circulating platelet levels.
• The protein is secreted and functions as a hormone, exerting its effects through the c-Mpl receptor on target cells.
• Structurally, it is a glycoprotein with disulfide bonds, and undergoes alternative splicing, resulting in multiple isoforms.
• Mutations in THPO are associated with inherited thrombocythemia and thrombocytopenia, highlighting its pivotal role in hematopoiesis.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated against a recombinant protein spanning a large portion of human THPO, likely recognizing multiple epitopes and native conformations.
• Predicted to detect a band around 38 kDa under reducing conditions in Western blotting; however, due to glycosylation, the observed molecular weight may be higher (approximately 60–70 kDa) in endogenous samples.
• Recommended to validate expression in relevant tissues like liver, kidney, or platelet-rich plasma.
• For ELISA applications, it can be used as a detection antibody; pairing with a capture antibody specific to a different epitope is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides ready-to-use IVD-grade antibodies, antigen raw materials, and supporting technical consultation. For research on thrombopoiesis, platelet disorders, or megakaryocyte biology, we supply validated antibody pairs, recombinant proteins, and ancillary reagents to support your assay development and diagnostic manufacturing.