Anti-THOP1 Rabbit Polyclonal Antibody for WB, ELISA - P52888

Core Product Specifications and Parameters

Biological Background: THOP1 Function and Localization

• Encodes thimet oligopeptidase (THOP1), also known as endopeptidase 24.15 or MP78, a zinc metalloprotease.
• Plays a critical role in the metabolism of neuropeptides shorter than 20 amino acid residues within the cytoplasm.
• Participates in cytoplasmic peptide degradation and can process the amyloid-beta precursor protein (APP), leading to the generation of amyloidogenic fragments. Related references: PMID:17251185, PMID:7639763
• Functions as a regulator of the cannabinoid signaling pathway by degrading hemopressin, an antagonist peptide of the cannabinoid receptor CNR1.
• Localizes predominantly to the cytoplasm.
• Contains phosphorylation and acetylation modifications, and features alternative splicing variants.
• Has a calculated molecular weight of approximately 78.8 kDa (UniProt), consistent with the observed ~79 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, validate the antibody in the relevant sample system; the expected band is ~79 kDa. Consider optimizing the primary antibody dilution to achieve optimal signal-to-noise ratio.
• The immunogen covers the N-terminal region (aa 1-260); ensure the target epitope is accessible under the chosen experimental conditions.
• When performing ELISA, titrate the antibody to determine the optimal working concentration for your specific assay format.
• Cross-reactivity is reported for mouse and rat samples; verify performance in these species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibodies, antigen pairs, and ancillary reagents to support neurobiology and Alzheimer's disease research. We facilitate procurement of optimized polyclonal antibodies like this anti-THOP1 product, alongside bulk reagents and technical consultation for diagnostic development. Whether you need a single antibody or a complete raw-material supply chain, CamelBio bridges the gap from concept to clinic.