Anti-THOC7 Polyclonal Antibody for WB, ELISA - Q6I9Y2

Core Product Specifications and Parameters

Biological Background: THOC7 Function and Localization

• THOC7 is a component of the THO subcomplex of the TREX complex, also known as fSAP24, hTREX30, and NIF3L1BP1.
• It couples mRNA transcription, processing, and nuclear export by selectively binding spliced mRNA (not unspliced pre-mRNA). Related references: PMID:15833825, PMID:15998806, PMID:17190602
• Plays a key structural role in the oligomerization of the THO-DDX39B complex. Related references: PMID:33191911
• Required for efficient export of polyadenylated RNA. Related references: PMID:23222130
• Recruited to spliced mRNAs upstream of the exon-junction complex in a splicing- and cap-dependent manner, facilitating export through the TAP/NXF1 pathway. Related references: PMID:15833825, PMID:15998806, PMID:17190602
• Localizes to the cytoplasm, nucleus, and nuclear speckles.
• Contains acetylation and phosphorylation sites, a coiled-coil domain, and is an RNA-binding protein.
• The TREX complex, including THOC7, is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-204), suggesting recognition of full-length THOC7; consider antigen retrieval or native conditions to preserve epitopes.
• For Western blot, the predicted molecular weight is ~24 kDa; validate in the relevant sample system (human, mouse, or rat) and confirm band specificity with siRNA knockdown or knockout controls.
• For ELISA, use the recombinant immunogen as a positive control and optimize coating concentrations.
• Since cross-reactivity spans human, mouse, and rat, this antibody may be suitable for comparative studies; verify species-specific reactivity in pilot experiments.
• THOC7 is part of a multi-protein complex; avoid harsh denaturing conditions that might disrupt epitope recognition; mild lysis buffers are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For investigations into mRNA processing and nuclear export, our THOC7 polyclonal antibody offers a reliable tool, supporting workflows from concept to clinic. Count on CamelBio to bridge your sourcing needs with scientific expertise.