Anti-THOC6 Polyclonal Antibody for WB - Q86W42

Core Product Specifications and Parameters

Biological Background: THOC6 Function and Localization

• THOC6 (THO complex subunit 6), also known as WDR58, fSAP35, or MMRFCGU, is a component of the THO subcomplex of the TREX (transcription/export) complex. Related references: PMID:15833825, PMID:15998806, PMID:17190602
• THOC6 plays a key structural role in the oligomerization of the THO-DDX39B complex, which is essential for coupling mRNA transcription, processing, and nuclear export. Related references: PMID:33191911
• The TREX complex is recruited to spliced mRNAs and facilitates their export to the cytoplasm via the TAP/NXF1 pathway in a splicing- and cap-dependent manner. Related references: PMID:15998806, PMID:17190602
• THOC6 contains WD repeats and is localized to the nucleus, particularly in nuclear speckles.
• The protein is involved in apoptosis negative control during brain development. Related references: PMID:15833825, PMID:23621916
• During microbial infection, the TREX complex is essential for the export of intronless mRNAs of Kaposi's sarcoma-associated herpesvirus (KSHV) and infectious virus production.
• Post-translational modifications include phosphorylation, and THOC6 is associated with mRNA processing and transport.

Experimental Guidance and Technical Tips

• For Western blot, consider using nuclear-enriched lysates to enhance detection due to THOC6's nuclear localization.
• Validate antibody specificity by using siRNA knockdown or CRISPR knockout controls, as the immunogen is a synthetic peptide.
• Optimize antibody dilution for your specific sample and detection system; recommended starting dilutions are not provided but can be empirically determined.
• In ELISA, test both direct and indirect coating methods and appropriate blocking buffers to minimize background.
• Cross-reactivity with mouse suggests potential utility in rodent model studies; always validate in the relevant sample context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. For mRNA processing and nuclear export research, we offer bulk sourcing of THOC6 antibodies and can assist with custom procurement for rare targets. Partner with CamelBio to streamline your assay development from concept to clinic.