Anti-THOC4/ALYREF Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q86V81

Core Product Specifications and Parameters

Biological Background: THOC4/ALYREF Function and Localization

• THOC4/ALYREF, also known as THO complex subunit 4, is a core component of the TREX (transcription/export) complex and functions as an mRNA export adapter, coupling transcription to mRNA processing and nuclear export of spliced mRNAs. Related references: PMID:15833825 PMID:15998806 PMID:17190602

• Binds specifically to spliced mRNA and is recruited in a cap- and splicing-dependent manner near the 5' end, interacting with the cap-binding protein NCBP1 to assemble the TREX complex. Related references: PMID:15833825 PMID:15998806 PMID:17190602

• Mediates nuclear export of intronless mRNAs by being recruited through ATP-bound DDX39B; this pathway is essential for the export of viral intronless mRNAs, such as those of Kaposi's sarcoma-associated herpesvirus (KSHV). Related references: PMID:17984224

• Acts as a bridging adaptor between the exon-junction complex (EJC) and the TREX core, facilitating the handover of mRNA to the NXF1-NXT1 export receptor for translocation through the nuclear pore complex. Related references: PMID:37020021 PMID:11675789 PMID:11707413 PMID:11979277

• Cooperates with THOC5 to enhance the RNA-binding activity of NXF1 and is required for its localization to the nuclear rim, thereby driving the nuclear export of specific mRNAs such as HSP70. Related references: PMID:19165146

• Recognizes 5-methylcytosine (m5C)-modified mRNAs and mediates their nucleo‑cytoplasmic shuttling, adding a layer of epitranscriptomic regulation to mRNA export. Related references: PMID:28418038

• Functions as a molecular chaperone that promotes the dimerization of basic leucine zipper (bZIP) transcription factors, thereby enhancing transcriptional activation. Related references: PMID:10488337

• Subcellularly localizes to the nucleus, nuclear speckles, and cytoplasm; post‑translational modifications include acetylation, citrullination, and phosphorylation. Expressed in a wide variety of cancer types, and also involved in transcription elongation and genome stability. Related references: PMID:12438613

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N‑terminal region (aa 1–100) of human THOC4/ALYREF. Verify sequence conservation in mouse and rat (UniProt accessions: Q8VE80, Q6AYK3) to ensure expected cross‑reactivity based on amino acid identity.
• For Western blot (WB), expect a band at ~27 kDa. Include lysates from HeLa, HEK293, or other THOC4‑expressing cells as positive controls; siRNA knockdown or knockout samples can serve as negative controls.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize antigen retrieval (e.g., heat‑induced epitope retrieval in citrate buffer) and permeabilization. THOC4/ALYREF exhibits nuclear and nucleoplasmic staining; co‑stain with nuclear markers to confirm localization.
• For ELISA applications, coat the plate with the immunizing peptide or recombinant THOC4/ALYREF protein. Titrate the antibody from 1:500 to 1:32,000 and detect with an appropriate secondary antibody.
• Always validate performance in the relevant sample system and application; recommended literature controls may be found in the cited publications.

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