Anti-THOC2 Rabbit Polyclonal Antibody for WB and ELISA - Q8NI27

Core Product Specifications and Parameters

Biological Background: THOC2 Function and Localization

• THOC2 is a core subunit of the THO subcomplex of the TREX complex, responsible for coupling mRNA transcription, processing, and nuclear export. Related references: PMID:15833825, PMID:15998806, PMID:17190602
• It directly interacts with DDX39B and is required for the efficient export of polyadenylated and spliced mRNAs. Related references: PMID:23222130, PMID:33191911
• THOC2 facilitates the nuclear rim localization of NXF1, a key mRNA export receptor. Related references: PMID:22893130
• The protein is involved in releasing mature mRNA from nuclear speckle domains.
• (Microbial infection) It is essential for the nuclear export of intronless mRNAs of Kaposi's sarcoma-associated herpesvirus (KSHV), required for infectious virus production.
• Subcellular localization: predominantly nuclear, concentrated in nuclear speckles, but also detected in the cytoplasm.
• Tissue specificity: enriched in the hippocampus and cerebral cortex, consistent with its links to intellectual disability (MRX12, MRX35, XLID12 as aliases).
• THOC2 undergoes alternative splicing and is classified under several functional keywords including phosphoprotein, RNA-binding, and mRNA transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-270) of human THOC2, suggesting recognition of full-length protein and potential N-terminal isoforms. For WB, consider using nuclear extracts and a reducing SDS-PAGE with high molecular weight markers (protein weight ~183 kDa).
• In ELISA, titrate the antibody against recombinant THOC2 or cell lysates to determine optimal working concentration; verify specificity with appropriate controls.
• Cross-reactivity with mouse THOC2 has been observed; validate in the relevant sample system if using mouse models.
• As a polyclonal antibody, evaluate lot-to-lot consistency when scaling up for IVD applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers and manufacturers working on mRNA biogenesis and nuclear export pathways, CamelBio offers consistent supply of the anti-THOC2 polyclonal antibody along with technical consultation to support your diagnostic development from concept to clinic.