Anti-Thiosulfate Sulfurtransferase (TST) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q16762

Core Product Specifications and Parameters

Biological Background: Thiosulfate Sulfurtransferase Function and Localization

• Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in sulfur transfer and cyanide detoxification.
• Catalyzes the transfer of a sulfur ion from thiosulfate to cyanide, forming thiocyanate; a key reaction in cyanide clearance (PubMed:40033006). Also exhibits weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Related references: PMID:40033006
• Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA; only the nascent unfolded cytoplasmic form binds 5S rRNA (PubMed:20663881, PubMed:21685364). Related references: PMID:20663881 PMID:21685364
• Localized to the mitochondrial matrix.
• Post-translationally modified by acetylation and phosphorylation; also reported as a glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the entire human TST sequence (aa 1–297), enabling recognition of multiple epitopes in denaturing and native assays.
• For Western blotting, a single band at ~33 kDa is anticipated; ensure mitochondrial protein extraction protocols are used.
• In immunohistochemistry (paraffin), antigen retrieval conditions may need optimization for mitochondrial targets.
• In immunoprecipitation, the antibody may capture native TST; validate in your sample system.
• For ELISA, coat with recombinant TST protein and employ standard polyclonal detection workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers and researchers a comprehensive sourcing platform for critical raw materials. This anti-TST polyclonal antibody supports mitochondrial and toxicology research, complementing our catalog of validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For customized or rare-target raw-material needs, CamelBio provides tailored procurement solutions from concept to clinic.