Anti-Thioredoxin reductase 2 (TXNRD2) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NNW7

Core Product Specifications and Parameters

Biological Background: Thioredoxin Reductase 2 Function and Localization

• TXNRD2 is a mitochondrial selenoprotein that plays a key role in controlling reactive oxygen species (ROS) levels and maintaining mitochondrial redox homeostasis by keeping thioredoxin in a reduced state; it may also participate in redox-regulated cell signaling (PubMed:24601690). Related references: PMID:24601690

• The enzyme uses FAD and NADP as cofactors to transfer reducing equivalents, functioning as an oxidoreductase with a redox-active center containing selenocysteine.

• TXNRD2 is localized to the mitochondrion, where it executes its redox-regulatory functions.

• The TXNRD2 gene produces multiple isoforms through alternative splicing.

• Tissue expression is highest in prostate, ovary, liver, testis, uterus, colon, small intestine, and adrenal cortex, with lower expression in brain, skeletal muscle, heart, and spleen; expression in kidney varies between reports.

• The protein is a member of the thioredoxin reductase family and is classified as a flavoprotein.

• Post-translational modifications include a selenocysteine residue essential for catalytic activity, and the protein undergoes mitochondrial import with a transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-310, a region that may be conserved across splicing isoforms; consider that the polyclonal antibody may recognize multiple TXNRD2 variants.
• For western blotting, a band at ~57 kDa is expected; however, isoform-specific migration may differ; validate in the relevant sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), confirm mitochondrial staining patterns with appropriate controls and antigen retrieval methods.
• Cross-reactivity with mouse is predicted, making this antibody suitable for rodent model studies; however, empirical validation is recommended.
• For ELISA applications, standard optimization of coating and detection conditions is necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions by providing one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-TXNRD2 polyclonal antibody, targeting a key mitochondrial redox regulator, aligns with our capability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for mitochondrial and oxidative stress research. Partner with us to streamline your raw material procurement and accelerate your assay development.