Anti-Thioredoxin Reductase 2 (TXNRD2) Rabbit Monoclonal Antibody for WB, ELISA - Q9NNW7

Core Product Specifications and Parameters

Biological Background: Thioredoxin Reductase 2 Function and Localization

• Thioredoxin reductase 2 (TXNRD2), also known as TR-beta, TR3, or Selenoprotein Z (SelZ), is a selenocysteine-containing member of the thioredoxin reductase family encoded by the TXNRD2 gene.
• TXNRD2 is a mitochondrial flavoprotein that utilizes NADPH, FAD, and a redox-active selenocysteine residue to maintain thioredoxin in a reduced state, playing a central role in controlling reactive oxygen species (ROS) levels and regulating mitochondrial redox homeostasis. Related references: PMID:24601690
• The enzyme is part of the pyridine nucleotide-disulfide oxidoreductase family and contains characteristic disulfide bonds and FAD-binding domains essential for its catalytic activity.
• TXNRD2 localizes predominantly to the mitochondria, consistent with its role in mitochondrial redox signaling and protection against oxidative stress.
• Tissue expression profiling reveals high TXNRD2 levels in prostate, ovary, liver, testis, uterus, colon, small intestine, and adrenal cortex, with intermediate expression in brain, skeletal muscle, heart, and spleen.
• Alternative splicing of TXNRD2 generates multiple transcript variants, which may contribute to functional diversity and tissue-specific regulation of mitochondrial thioredoxin reductase activity.
• Post-translational modifications include a transit peptide for mitochondrial import and the co-translational incorporation of selenocysteine, which is critical for the enzyme's redox function.
• Genetic variants and disease mutations in TXNRD2 have been linked to human disorders, such as GCCD5 (glucocorticoid deficiency 5), underscoring its importance in adrenal function and stress response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human TXNRD2. When performing western blotting, a band near 57 kDa is expected; slight shifts due to mitochondrial transit peptide cleavage may be observed.
• For ELISA applications, direct or sandwich formats may be considered, but optimal working concentrations should be determined empirically in the user’s assay system.
• Cross-reactivity is reported for human, mouse, and rat. When working with these species, validate the antibody in the relevant tissue or cell lysate, as expression levels can vary (high in prostate, liver, etc.).
• Due to the presence of selenocysteine, confirm that the detection system does not interfere with the redox-sensitive epitope; reducing conditions are typically compatible with standard WB protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner specializing in providing diagnostic manufacturers, labs, and research institutes with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. The Anti-TXNRD2 antibody supports research in mitochondrial biology, oxidative stress, and redox signaling. By leveraging CamelBio’s comprehensive supply chain, customers can accelerate development from concept to clinic.