Anti-Thioredoxin 2 (TXN2) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q99757

Core Product Specifications and Parameters

Biological Background: Thioredoxin 2 (TXN2) Function and Localization

• Thioredoxin 2 (TXN2) is a mitochondrial member of the thioredoxin family, encoded by the TXN2 gene, with a molecular weight of approximately 18 kDa (UniProt: 18383 Da) and a transit peptide for mitochondrial targeting.
• TXN2 plays a critical role in mitochondrial reactive oxygen species (ROS) homeostasis, apoptosis regulation, and maintenance of cell viability (PubMed:12032145, PubMed:12080052, PubMed:26626369). Related references: PMID:12032145 PMID:12080052 PMID:26626369
• It catalyzes reversible oxidation of its active center dithiol to a disulfide, mediating thiol-disulfide exchange and the reduction of protein disulfide bonds in the mitochondria (By similarity).
• TXN2 is localized exclusively to the mitochondria, consistent with its role in mitochondrial redox signaling.
• Widely expressed across adult and fetal tissues, indicating a fundamental role in cellular metabolism.
• Post-translational modifications include acetylation, and its activity depends on a redox-active disulfide bond center; it is associated with conditions such as neurodegeneration and primary mitochondrial disease (keywords).

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a synthetic peptide spanning amino acids 67–166 of human TXN2, targeting the mature protein sequence beyond the mitochondrial transit peptide.
• WB Application: For Western blot, consider using mitochondrial‑enriched lysates and reducing conditions to resolve the ~18 kDa band. Validate in relevant human, mouse, or rat cell lines.
• IF/ICC: Perform immunofluorescence after appropriate permeabilization; include a mitochondrial marker for co‑localization to confirm specificity.
• ELISA: Can be used for detection of TXN2 in capture or detection formats; optimize coating and blocking conditions for the assay matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw materials, offering validated antibodies, antigen pairs, and ancillary reagents to accelerate diagnostic development. This anti‑TXN2 monoclonal antibody supports research into mitochondrial disorders and redox biomarkers, with custom sourcing available for bulk orders and rare targets. From concept to clinic, CamelBio bridges your procurement needs with quality‑assured biological materials.