Anti-Thioredoxin 1 (Trx1/TXN) Monoclonal Antibody for WB, IHC-P, ELISA - P10599

Core Product Specifications and Parameters

Biological Background: Thioredoxin 1 (Trx1/TXN) Function and Localization

• Thioredoxin 1 (TXN), also known as ATL-derived factor (ADF) or surface-associated sulphydryl protein (SASP), is a multifunctional redox-active protein encoded by the TXN gene.
• Catalyzes dithiol-disulfide exchange reactions through reversible oxidation of its active center dithiol to a disulfide. Related references: PMID:17182577 PMID:19032234 PMID:2176490
• Mediates reversible S-nitrosylation of cysteine residues in target proteins; nitrosylates the active site cysteine of CASP3, inhibiting caspase-3 activity in response to nitric oxide. Related references: PMID:16408020 PMID:17606900
• Induces FOS/JUN AP-1 DNA-binding activity and stimulates AP-1 transcriptional activity in ionizing radiation-treated cells. Related references: PMID:11118054 PMID:9108029
• ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
• Localizes to the nucleus, cytoplasm, and can be secreted from cells.
• Post-translational modifications include acetylation, disulfide bond formation, S-nitrosylation, and ubiquitination; serves as an electron transport and transcription regulation factor.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning amino acids 26-105 of human TXN, targets a conserved region across human, mouse, and rat, enabling the observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 12 kDa; consider using a 12-15% gel and optimizing transfer conditions for low molecular weight proteins.
• In immunohistochemistry (IHC-P), ensure proper antigen retrieval and validate staining patterns in relevant tissue samples to minimize non-specific binding.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate to determine optimal working concentration in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner for IVD raw materials, streamlining the transition from concept to clinic. For researchers and manufacturers studying redox biology, oxidative stress, or apoptosis, we offer a reliable supply of validated antibodies like this Thioredoxin 1 monoclonal antibody, complemented by bulk ancillary reagents, optimized antibody pairs, and sourcing for rare target raw materials to accelerate diagnostic development.