Anti-THEMIS2 Polyclonal Antibody for WB, ELISA - Q5TEJ8

Core Product Specifications and Parameters

Biological Background: THEMIS2 Function and Localization

• Protein THEMIS2, also known as ICB-1 or Thymocyte-expressed molecule involved in selection protein 2, is encoded by the THEMIS2 gene (C1orf38, ICB1).
• It functions as a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Related references: PMID:20644716
• Determines the threshold for B cell activation by low-affinity and low-avidity ligands through PLCG2 activation and its downstream pathways.
• Localizes to both the nucleus and cytoplasm.
• Expressed in various endometrial adenocarcinoma cell lines and multiple other cell lines, but not in prostate LNCaP or ovarian cancer BG1 cells.
• Classified as an alternative splicing product and a phosphoprotein involved in immunity and inflammatory response.
• The predicted molecular weight is approximately 72 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–260), enabling recognition of full-length and potentially N-terminal isoforms.
• Suitable for western blot (WB) and ELISA; consider using denaturing/reducing conditions for WB given the ~72 kDa size.
• Validate detection in your specific sample system, particularly macrophage or B cell models where THEMIS2 is functionally relevant.
• Cross-reactivity with mouse and rat is reported; consider using appropriate secondary antibodies and lysates for these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. This THEMIS2 polyclonal antibody serves immunology and inflammation research, and CamelBio can supply validated antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development and clinical transition.