Anti-THBS2 Polyclonal Antibody for WB, ELISA - P35442

Core Product Specifications and Parameters

Biological Background: THBS2 Function and Localization

• Thrombospondin-2 (THBS2), also known as TSP2, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
• It serves as a ligand for CD36, thereby mediating antiangiogenic properties.
• THBS2 shows high expression in intervertebral disk tissue.
• The protein contains structural features including EGF-like domains, calcium-binding sites, and multiple repeat regions.
• Post-translational modifications include glycosylation and formation of disulfide bonds, and the protein has a signal peptide for secretion.
• THBS2 is associated with disease variants, including Ehlers-Danlos syndrome, and contains heparin-binding motifs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 20-280) of human THBS2, which may contain functionally relevant domains; consider its suitability for detecting THBS2 in various sample types.
• For western blotting, the predicted molecular weight is ~130 kDa; use appropriate gel percentages and transfer conditions, and validate the antibody with positive control lysates from human, mouse, or rat tissues, especially those with high THBS2 expression such as intervertebral disk.
• As a polyclonal antibody, consider potential lot-to-lot variability; optimize dilution factors and perform appropriate controls for each new application.
• For ELISA, ensure proper coating buffers and detection systems are optimized, and validate specificity using recombinant or purified THBS2 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on cell adhesion or anti-angiogenesis pathways, we offer reliable supply solutions for THBS2 antibodies, including validated polyclonal and monoclonal formats, as well as bulk ancillary reagents. Our comprehensive sourcing capabilities ensure you can obtain high-quality raw materials for your THBS2-related assay development and validation needs.