Anti-THBS1 Rabbit Monoclonal Antibody for WB - P07996

Core Product Specifications and Parameters

Biological Background: Thrombospondin-1 Function and Localization

• THBS1, also known as Glycoprotein G, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  Related references: PMID:15014436 PMID:18285447 PMID:2430973
• Multifunctional protein involved in inflammation, angiogenesis, wound healing, ROS signaling, NO signaling, apoptosis, senescence, aging, stem cell self-renewal, and metabolic homeostasis.
  Related references: PMID:10613822 PMID:11134179 PMID:1371676
• Negatively modulates dendritic cell activation and cytokine release, contributing to the resolution of inflammation and immune homeostasis.
  Related references: PMID:14568985
• Ligand for receptors CD47, CD36, and SIRPA, mediating effects on NO signaling, ROS production, cellular senescence, apoptosis, and angiogenesis suppression.
  Related references: PMID:19004835 PMID:8550562 PMID:29042481 PMID:10613822 PMID:11134179 PMID:1371676 PMID:24511121
• May play a role in metabolic dysfunction in diet-induced obesity and ER stress response via interactions with ATF6.
• Subcellular localization includes secretion, cell surface, extracellular matrix, endoplasmic reticulum, and sarcoplasmic reticulum.
• Tissue specificity: Expressed by platelets (at protein level) and by monocyte-derived immature and mature dendritic cells (at protein level).
  Related references: PMID:101549 PMID:14568985
• Keywords: Angiogenesis, Apoptosis, Calcium, Cell adhesion, Disulfide bond, EGF-like domain, Endoplasmic reticulum, Extracellular matrix, Glycoprotein, Heparin-binding, Inflammatory response, Secreted, Signal, Unfolded protein response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-34 of human THBS1. For Western blot, consider using reducing conditions, as THBS1 contains disulfide bonds; the observed band may appear at approximately 120 kDa (reducing) or at the full-length 129 kDa.
• Validate the antibody in relevant human, mouse, or rat samples, such as platelet lysates or dendritic cell extracts.
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval may be necessary to expose the epitope.
• Optimize antibody dilution for IP and ELISA by titration in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-THBS1 monoclonal antibody supports research into inflammation, angiogenesis, and metabolic disorders. CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.