Anti-THAP2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H0W7

Core Product Specifications and Parameters

Biological Background: THAP2 Function and Localization

• THAP2 belongs to the THAP (Thanatos-associated protein) domain-containing protein family, characterized by a conserved zinc finger motif that facilitates sequence-specific DNA binding.
• The protein contains a C2CH-type zinc finger, enabling it to coordinate a zinc ion essential for proper folding and function.
• THAP2 is annotated as a DNA-binding protein and participates in transcriptional regulation through its interaction with chromatin and promoter regions.
• Proteomics identification and inclusion in the human reference proteome indicate its ubiquitous expression and conservation across samples.
• The molecular weight of THAP2 is predicted to be 26 kDa, consistent with its observed band in experimental settings.
• Three-dimensional structural data (keyword: 3D-structure) provide insights into the molecular architecture of the THAP domain and its DNA recognition mechanism.
• Metal-binding properties, particularly to zinc, are critical for the structural integrity and regulatory function of THAP2.
• As a reference proteome member, THAP2 serves as a benchmark for proteomic studies and antibody validation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 83–228 of human THAP2, covering a region likely encompassing part of the THAP domain; verify that the antibody detects the endogenous protein at the predicted molecular weight of 26 kDa in whole cell lysates.
• For Western blot, consider using reducing conditions and an appropriate loading control, and confirm specificity through siRNA knockdown or overexpression experiments in relevant human cell lines.
• In ELISA applications, the polyclonal nature may enhance signal amplification; optimize coating and detection antibody concentrations for your target antigen.
• Cross-reactivity with mouse and rat suggests conserved epitopes; validate reactivity and off-target binding in these species if used in rodent models.
• As a polyclonal antibody, expect lot-to-lot variability; routine validation with your experimental system is recommended to ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing reliable access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The THAP2 Rabbit pAb targets a zinc-finger DNA-binding protein with applications in chromatin and transcription research, supporting assay development needs. For rare targets or custom requirements, CamelBio facilitates procurement of specialized raw materials, leveraging its global supply network to streamline your product development pipeline.