Anti-THADA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6YHU6

Core Product Specifications and Parameters

Biological Background: THADA Function and Localization

• THADA (Thyroid adenoma-associated protein), also designated GITA, ARMC13, and Trm732, is a regulatory subunit of the tRNA methyltransferase complex.
• Together with the methyltransferase FTSJ1, THADA directs 2'-O-ribose methylation of nucleotide 32 in the anticodon loop of substrate tRNAs, a modification critical for translation fidelity.
• THADA exhibits broad expression in endocrine and gastrointestinal tissues, including pancreas, adrenal medulla and cortex, thyroid, testis, thymus, small intestine, and stomach.
• THADA is implicated in thyroid adenomas through chromosomal rearrangements, as indicated by its alias 'Gene inducing thyroid adenomas protein'.
• THADA is a phosphoprotein with multiple phosphorylation sites identified by proteomic studies, suggesting regulated activity.
• Alternative splicing of THADA transcripts generates multiple isoforms, potentially contributing to functional diversity.
• The full-length protein has a predicted molecular weight of approximately 220 kDa (219.6 kDa from UniProt), consistent with the product validation.

Experimental Guidance and Technical Tips

• For Western blotting, use a low-percentage (e.g., 6-8%) polyacrylamide gel to resolve the 220 kDa THADA protein effectively.
• In immunofluorescence, validate antibody specificity in cell lines or tissues with documented THADA expression, such as thyroid or pancreatic tissue.
• For ELISA, consider using the recombinant immunogen fragment as a positive control to assess binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-THADA reagent. Our portfolio supports tRNA modification research and thyroid disease biomarkers with bulk ancillary reagents and custom sourcing for rare targets.