Anti-TGS1 Rabbit Polyclonal Antibody for WB and ELISA - Q96RS0

Core Product Specifications and Parameters

Biological Background: TGS1 Function and Localization

• TGS1, also known as PIMT, PIPMT, or NCOA6IP, is a trimethylguanosine synthase that catalyzes the two-step hypermethylation of the 7-monomethylguanosine (m7G) cap of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m2,2,7)G cap structure.
• The enzyme is specific for guanine and requires N7 methylation before N2 methylation, using S-adenosyl-L-methionine as a methyl donor.
• Hypermethylation of the m7G cap by TGS1 promotes the concentration of U snRNAs in nuclear foci, their colocalization with coilin, and the formation of canonical Cajal bodies (CBs).
• Beyond snRNA processing, TGS1 plays a role in transcriptional regulation as a nuclear receptor coactivator 6-interacting protein.
• Immunofluorescence and subcellular fractionation studies localize TGS1 to the cytoplasm, nucleus, Cajal bodies, and nucleolus.
• TGS1 is ubiquitously expressed with high levels detected in heart, skeletal muscle, kidney, liver, and placenta.
• As a phosphoprotein and methyltransferase, TGS1 is subject to post-translational modification that may regulate its activity or localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 714–853 of human TGS1, which may target a region involved in protein–protein interactions or substrate binding; thus, confirm reactivity in your system.
• For Western blot, a band around 97 kDa is expected based on the predicted molecular weight; validate using positive controls from human or mouse cell lysates.
• While validated for ELISA and WB, the antibody may also be suitable for other applications such as immunoprecipitation or immunofluorescence; consider pilot optimization.
• Given the polyclonal nature and cross-reactivity with mouse TGS1, this antibody can be a versatile tool for studying TGS1 in multiple species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, supporting diagnostic manufacturers, laboratories, and research institutes. For scientists investigating RNA processing, Cajal body dynamics, or transcriptional regulation, this anti-TGS1 polyclonal antibody streamlines assay development. Let CamelBio be your reliable partner for sourcing even rare-target reagents, ensuring you have the materials needed from concept to clinic.