Anti-TGN46/TGOLN2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43493

Core Product Specifications and Parameters

Biological Background: TGN46/TGOLN2 Function and Localization

• The gene TGOLN2 encodes multiple isoforms including TGN46, TGN48, and TGN51, collectively known as trans-Golgi network integral membrane proteins.
• TGOLN2 proteins are thought to participate in regulating membrane traffic between the trans-Golgi network and other compartments.
• TGN46 is widely expressed across tissues, whereas TGN51 is enriched in fetal lung and kidney, and TGN48 shows very limited expression.
• Subcellularly, these proteins localize primarily to the trans-Golgi network membrane and can also be detected at the cell membrane.
• The protein is glycosylated and phosphorylated, indicating potential regulation by post-translational modifications.
• It contains a signal peptide, a transmembrane domain, and luminal repeats, characteristic of type I membrane proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 323-423) of human TGN46/TGOLN2; check sequence alignment in mouse and rat to assess expected cross-reactivity.
• For Western blot, a band around 46 kDa is expected; optimal antibody dilution should be determined empirically.
• In immunohistochemistry (paraffin), antigen retrieval may enhance signal; validate with known positive tissues such as those with high trans-Golgi network content.
• For ELISA, the antibody can be used for detection; pairing with a suitable capture antibody may be considered for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, offering validated antibodies, assay-ready reagents, and custom sourcing for targets like TGN46/TGOLN2, which is critical for studying membrane trafficking. We support diagnostic kit developers with bulk monoclonal/polyclonal antibodies and ancillary raw materials, streamlining the path from discovery to clinical application.