Anti-TGN46/TGOLN2 Monoclonal Antibody for WB, IHC-P, ELISA - O43493

Core Product Specifications and Parameters

Biological Background: TGN46/TGOLN2 Function and Localization

• TGN46 (Trans-Golgi network integral membrane protein 2), also known as TGOLN2, is a transmembrane glycoprotein of the trans-Golgi network (TGN) encoded by the TGOLN2 gene. Alternative splicing produces isoforms including TGN46, TGN48, and TGN51.
• It is suggested to play a role in regulating membrane trafficking to and from the trans-Golgi network, acting as a TGN marker.
• TGN46 localizes primarily to the trans-Golgi network membrane and cycles between the TGN and the cell surface, marking the late secretory pathway.
• Isoform TGN46 is widely expressed in human tissues, whereas TGN51 is enriched in fetal lung and kidney, and TGN48 shows low expression in embryonic kidney and promyelocytic cells.
• The protein undergoes post-translational modifications including N-linked glycosylation and phosphorylation, and contains a signal peptide, a transmembrane helix, and multiple repeat regions.
• Keywords associated with TGN46 include alternative splicing, glycoprotein, phosphoprotein, and Golgi apparatus, highlighting its dynamic regulation and organelle-specific function.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the N-terminal region (aa 1-100) of human TGN46; verify epitope accessibility in your target species and sample type.
• For Western blotting, the predicted molecular weight is approximately 46 kDa; consider using appropriate gel percentages and loading controls.
• In immunohistochemistry (IHC), optimize antigen retrieval and permeabilization steps to achieve consistent staining of the Golgi compartment.
• For ELISA applications, validate antibody binding under relevant buffer and coating conditions with the target peptide or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic developers and researchers. For studies involving Golgi markers such as TGN46, CamelBio can assist in procuring rare target antibodies and related reagents, streamlining the path from assay concept to clinical application. With a focus on trans-Golgi network trafficking and organelle research, CamelBio supports your project with tailored procurement solutions and technical expertise.