Anti-TGM5 Polyclonal Antibody for WB, IHC-P, ELISA - O43548

Core Product Specifications and Parameters

Biological Background: TGM5 Function and Localization

• TGM5 (Transglutaminase 5) is a member of the transglutaminase enzyme family, known by multiple aliases including TGX, TGase X, and TGASEX.
• It catalyzes the calcium-dependent cross-linking of proteins and the conjugation of polyamines to proteins, playing an essential role in the formation of the cornified cell envelope of keratinocytes.
• The protein is localized in the cytoplasm and is predominantly expressed in foreskin keratinocytes, indicating its importance in epidermal differentiation.
• TGM5 undergoes alternative splicing, and its activity is regulated by post-translational modifications such as acetylation.
• As a metal-binding acyltransferase, TGM5 is associated with disease variants, highlighting its significance in skin barrier integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 461-720) of human TGM5, which may aid in detecting full-length and alternatively spliced isoforms.
• For Western blotting, expect a band at approximately 81 kDa; verify with molecular weight markers and optimize blocking and washing conditions.
• In immunohistochemistry (paraffin sections), consider heat-induced antigen retrieval and titrate the primary antibody to determine the optimal working concentration.
• Since the antibody cross-reacts with mouse and rat TGM5, it can be tested in rodent skin models; validate species-specific performance in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to TGM5 antibodies and ancillary IVD raw materials for keratinocyte envelope and epidermal barrier studies. Our integrated platform supplies validated rabbit polyclonal antibodies, bulk monoclonal antibodies, and customized procurement of rare-target reagents—facilitating your transition from research to clinical application with reliable, one-stop support.