Anti-TGM3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q08188

Core Product Specifications and Parameters

Biological Background: TGM3 Function and Localization

• TGM3 (Transglutaminase 3) functions as a calcium-dependent acyltransferase, catalyzing the formation of covalent isopeptide cross-links between protein-bound glutamine and lysine residues, as well as polyamine conjugation (By similarity).
• In terminally differentiating keratinocytes, TGM3 cross-links small proline-rich proteins (SPRR1, SPRR2) and loricrin into small interchain oligomers, which are subsequently incorporated into the cornified envelope scaffold by TGM1 (By similarity).
• Within hair follicles, TGM3-mediated cross-linking of structural proteins is essential for hardening the inner root sheath, contributing to hair strength.
• TGM3 is predominantly cytoplasmic and requires calcium binding for activation; it is synthesized as a zymogen that undergoes proteolytic cleavage to expose the active site.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and is classified under metal-binding, transferase, and keratinization-related protein families.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 291-480 of human TGM3, a region that may be partially conserved across species; cross-reactivity with mouse should be validated if used with mouse samples.
• For Western blot (WB) analysis, consider using reducing conditions and loading a positive control such as keratinocyte lysates; the predicted band is approximately 76 kDa.
• Immunofluorescence/immunocytochemistry (IF/ICC) may require permeabilization to detect cytoplasmic TGM3; validate antibody specificity with blocking peptides or appropriate controls.
• ELISA performance has been confirmed with the supplied antibody; ensure optimal coating antigen and detection system conditions are established by the end user.
• Due to the polyclonal nature, lot-to-lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies against skin and hair differentiation targets like TGM3. From routine polyclonal antibodies to customized bulk reagent sourcing, we support diagnostic manufacturers and research labs in accelerating assay development for keratinization, dermatopathology, and beyond. Our technical team can assist with material selection and procurement to streamline your workflow.