Anti-TGM3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q08188

Core Product Specifications and Parameters

Biological Background: TGM3/Transglutaminase 3 Function and Localization

• Transglutaminase 3 (TGM3), also designated TGase E, TG(E), TGE, or UHS2, is a calcium-dependent enzyme belonging to the transglutaminase family.
• TGM3 catalyzes the formation of isopeptide cross-links between glutamine and lysine residues in proteins, as well as the conjugation of polyamines to proteins, modifying protein structure and stability.
• It is essential for the formation of the cornified envelope (CE) in terminally differentiated keratinocytes, where it cross-links small proline-rich proteins (SPRR1 and SPRR2) and loricrin (LOR) into oligomers that are subsequently assembled by TGM1 onto the growing CE scaffold.
• In hair follicles, TGM3 contributes to the hardening of the inner root sheath by cross-linking structural proteins, a process critical for hair shaft integrity and strength.
• The protein localizes primarily to the cytoplasm and is subject to post-translational modifications such as acetylation and phosphorylation; it is produced as a zymogen requiring proteolytic cleavage for activation.
• UniProt annotations also note that TGM3 is a metal-binding acyltransferase with a known 3D structure, and it has been identified in proteomic analyses (keywords: Acetylation, Phosphoprotein, Zymogen, 3D-structure).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning the N-terminal region (amino acids 1-180) of human TGM3, which is expected to recognize full-length TGM3 and potentially splice variants that include this segment.
• For Western blotting (WB), the predicted molecular weight is ~77 kDa; using a 4-20% gradient gel or 8% SDS-PAGE is recommended. Include appropriate positive controls (e.g., keratinocyte lysates) and validate antibody specificity in relevant samples.
• In immunofluorescence/immunocytochemistry (IF/ICC), since TGM3 is cytoplasmic, consider optimising cell fixation and permeabilization conditions to ensure adequate antibody penetration while preserving antigenicity.
• For ELISA applications, the antibody can serve as a detection or capture reagent after confirming its performance with purified antigen or biological samples; validate in the relevant sample matrix.
• The antibody is reactive with both human and mouse TGM3, as per supplier data; validate cross-reactivity in your model system of interest before quantitative assays.
• Always titrate the antibody for each application and include proper controls to rule out non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research institutions, offering one-stop access to raw materials, technical services, and consulting across the diagnostic development pipeline. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support assays targeting skin biology and cornification markers like TGM3. Whether you need this anti-TGM3 polyclonal antibody or custom research-use reagents, CamelBio streamlines procurement from concept to clinic.