Anti-TGM1 Rabbit Polyclonal Antibody for WB, ELISA - P22735

Core Product Specifications and Parameters

Biological Background: TGM1 Function and Localization

• TGM1, also known as protein-glutamine gamma-glutamyltransferase K, is a transglutaminase enzyme encoded by the TGM1 gene.
• It catalyzes calcium-dependent cross-linking of proteins and conjugation of polyamines to proteins, essential for the formation of the stratum corneum. Related references: PMID:7629111, PMID:8824274, PMID:26220141
• TGM1 activity is also involved in the regulation of cell proliferation. Related references: PMID:26220141
• It localizes to the cell membrane, cytoplasm, and cytosol.
• TGM1 is predominantly expressed in the epidermis, where it plays a pivotal role in keratinocyte cornification.
• The protein undergoes alternative splicing and is post-translationally modified by palmitoylation and phosphorylation.
• Mutations in TGM1 are linked to autosomal recessive congenital ichthyosis (ARCI), a skin disorder characterized by abnormal keratinization.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100-280 of human TGM1, a region that may contribute to cross-reactivity with mouse and rat orthologs; confirm reactivity in your specific model system.
• For Western blotting, optimize antibody dilution and detection conditions for a ~90 kDa target; consider using a positive control such as epidermal or keratinocyte lysates.
• In ELISA applications, validate the antibody with recombinant TGM1 or native protein from relevant tissue extracts to establish optimal coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner for IVD raw materials, CamelBio supports diagnostic manufacturers and research labs with validated antibodies and bulk ancillary reagents for skin barrier and keratinization targets. Our portfolio includes options for rare target sourcing, customized antibody production, and matched antibody pairs to help accelerate your assay development for ichthyosis and related disorders. Contact us to discuss your specific procurement needs.