Anti-TGIF1 Polyclonal Antibody for WB, IHC-P, ELISA - Q15583

Core Product Specifications and Parameters

Biological Background: TGIF1 Function and Localization

• Homeobox protein TGIF1 (5'-TG-3'-interacting factor 1) functions as a transcriptional repressor.
• Binds to the RXR responsive element from the CRBPII promoter, inhibiting 9-cis-retinoic acid-dependent RXRα transcription activation.
• Acts as an active transcriptional corepressor of SMAD2, linking the nodal signaling pathway to forebrain bifurcation and ventral midline establishment.
• May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of RXRα activities.
• Subcellular localization is restricted to the nucleus (UniProt annotation).
• Features a homeobox DNA-binding domain; associated with holoprosencephaly and alternative splicing; part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250-340 of human TGIF1 (NP_733796.2). For cross-reactive species (mouse, rat), verify sequence homology before use.
• This polyclonal antibody has been used in WB, IHC-P, and ELISA. Optimal working dilutions and incubation conditions should be determined empirically.
• Because TGIF1 is a nuclear transcriptional repressor, consider nuclear extraction or appropriate fixation/permeabilization protocols for detection.
• For IHC-P, antigen retrieval steps may be necessary; validate in appropriate tissue samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials. For transcription regulation and developmental biology studies, we offer validated antibodies like this anti-TGIF1 polyclonal antibody, alongside optimized antibody pairs, recombinant proteins, and ancillary reagents. Our one-stop platform supports your project from concept to clinic with reliable raw-material sourcing.