Anti-TGFBI (TGF beta induced) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15582

Core Product Specifications and Parameters

Biological Background: TGFBI Function and Localization

• TGFBI encodes transforming growth factor-beta-induced protein ig-h3, also known as kerato-epithelin and RGD-CAP, which is a secreted extracellular matrix protein.
• The protein mediates cell adhesion through interactions with integrins and other matrix components, contributing to cell-extracellular matrix communication (PubMed:8024701). Related references: PMID:8024701
• It may also play a role in cell-collagen interactions, facilitating tissue organization and integrity.
• TGFBI is highly expressed in the corneal epithelium, as well as in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas (PubMed:27609313, PubMed:8077289). Related references: PMID:27609313 PMID:8077289
• Mutations in TGFBI are linked to diverse corneal dystrophies (e.g., granular, lattice, Avellino types), emphasizing its essential role in preserving corneal transparency.
• The protein undergoes gamma-carboxylation and phosphorylation, modifications that may influence its function and stability.
• Subcellularly, TGFBI is secreted into the extracellular space and matrix, consistent with its roles in cell adhesion and matrix assembly.

Experimental Guidance and Technical Tips

• The immunogen (a synthetic peptide spanning amino acids 50–150) targets a conserved region; cross-reactivity with human, mouse, and rat TGFBI is expected, but validation in the relevant species is recommended.
• For western blot (WB), a band around 75 kDa (or 74.7 kDa based on UniProt) is anticipated; use appropriate loading controls and consider reducing conditions.
• In immunohistochemistry (IHC-P), antigen retrieval steps (e.g., heat-mediated citrate buffer) may improve detection; include isotype controls and optimize antibody concentration.
• For ELISA, pairing this antibody with a suitable detection antibody can establish a sandwich assay; empirical determination of capture/detection concentrations is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For researchers focused on TGFBI—a key player in cell adhesion, corneal dystrophy, and extracellular matrix biology—we supply high-quality reagents to accelerate your assay development from concept to clinic. Let our technical and procurement experts streamline your supply chain for this target and beyond.