Anti-TGFB2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P61812

Core Product Specifications and Parameters

Biological Background: TGFB2 Function and Localization

• Encodes the transforming growth factor beta-2 proprotein, which is cleaved into Latency-associated peptide (LAP) and the active Transforming growth factor beta-2 chain.
• TGF-beta-2 is a multifunctional cytokine that regulates processes such as angiogenesis and heart development. Related references: PMID:22772368 PMID:22772371
• The mature TGF-beta-2 is maintained in a latent state through non-covalent association with LAP and interactions with 'milieu molecules' like LTBP1 and LRRC32/GARP in the extracellular matrix.
• Active TGF-beta-2 signals by binding to TGF-beta receptors (TGFBR1 and TGFBR2).
• Localizes to the extracellular space and extracellular matrix; it is secreted.
• Contains a signal peptide, is glycosylated, and features disulfide bonds; belongs to the TGF-beta family.
• Alternative splicing yields multiple isoforms; the proprotein undergoes cleavage at pairs of basic residues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–160 of the human TGFB2 proprotein (NP_001129071.1), which covers part of the LAP region. This polyclonal antibody should recognize both the proprotein and, potentially, the mature ligand after cleavage.
• For Western blotting (WB), consider running samples under reducing conditions and using a suitable positive control such as recombinant TGFB2 or cell lysates known to express TGFB2.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may enhance detection; validate with known TGFB2-positive tissue sections.
• For ELISA, the antibody can be explored as a capture or detection reagent; pairing with another clone or antibody may be necessary for sandwich ELISA development.
• Cross-reactivity has been confirmed only for human; verify performance in your species or sample system if extended beyond human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your partner for streamlined access to IVD raw materials, technical services, and consultancy—from concept to clinic. This anti-TGFB2 polyclonal antibody supports research into TGF-beta signaling pathways in angiogenesis and cardiac biology. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, all aligned with your diagnostic manufacturing and assay development workflows.