Anti-TGFB2 Rabbit Polyclonal Antibody for WB and ELISA - P61812

Core Product Specifications and Parameters

Biological Background: TGFB2 Function and Localization

• TGFB2 encodes the transforming growth factor beta-2 proprotein, which is cleaved into the Latency-associated peptide (LAP) and the mature TGF-beta-2 chain. LAP maintains TGF-beta-2 in a latent state during storage in the extracellular matrix (By similarity).
• TGF-beta-2 is a multifunctional growth factor that regulates key processes including angiogenesis and heart development, as demonstrated in loss-of-function studies (PubMed:22772368, PubMed:22772371). Related references: PMID:22772368 PMID:22772371
• Activation of TGF-beta-2 occurs when LAP is released, allowing the mature cytokine to bind to TGFBR1 and TGFBR2 receptors and transduce intracellular signals (By similarity).
• The protein is secreted and predominantly localized to the extracellular space and extracellular matrix (By similarity).
• Post‑translational modifications include cleavage on a pair of basic residues, disulfide bond formation, and glycosylation, which are critical for proper folding and latency regulation (By similarity).
• Alternative splicing of TGFB2 yields multiple isoforms, and mutations are associated with diseases such as aortic aneurysm.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–160 of human TGFB2, a region that spans part of the proprotein. For Western blotting, consider using reducing conditions and validate in lysates from cell lines or tissues known to express TGFB2 (e.g., tumor cell lines). The predicted molecular weight of the proprotein is 48 kDa, but cleavage products may also be detected.
• For ELISA applications, consider coating with recombinant TGFB2 protein or using sandwich formats with matched antibody pairs. Optimize concentrations experimentally.
• Because the antibody shows cross-reactivity with mouse and rat TGFB2, it is suitable for comparative studies; however, validate specificity in the respective species as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. For researchers studying TGF‑β signaling, angiogenesis, and cardiac development, CamelBio bridges the gap from concept to clinic with reliable supply and technical support.