Anti-TGFB1I1 Polyclonal Antibody for WB, ELISA - O43294

Core Product Specifications and Parameters

Biological Background: TGFB1I1 Function and Localization

• TGFB1I1, also known as Hic-5/ARA55, is a multifunctional adapter protein that coordinates protein-protein interactions at focal adhesions and in the nucleus, linking signaling modules to plasma membrane receptors.
• It regulates the Wnt and TGFB signaling pathways and may influence neurotransmitter transporter targeting (SLC6A3 and SLC6A4) to the plasma membrane.
• In the nucleus, TGFB1I1 acts as a nuclear receptor coactivator for glucocorticoid, androgen, mineralocorticoid, and progesterone receptors, modulating their transcriptional activity.
• The protein contains LIM domains and a zinc-binding motif, suggesting a structural basis for its DNA-binding activity and protein interactions.
• Subcellular localization includes focal adhesions, nuclear matrix, and the cytoskeleton, reflecting its dynamic roles in cell adhesion and transcriptional regulation.
• Tissue-specific expression is observed in platelets, smooth muscle, and prostate stromal cells, indicating roles in cardiovascular and reproductive systems.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.
• It is implicated in key cellular processes such as growth, proliferation, migration, differentiation, and senescence.

Experimental Guidance and Technical Tips

• For WB, consider using whole-cell lysates or nuclear extracts from human, mouse, or rat cell lines; the predicted protein weight is ~50 kDa, but note possible post-translational modifications.
• For ELISA, validate the antibody with recombinant TGFB1I1 protein or peptide as a positive control.
• Due to its role in focal adhesions and nuclear matrix, subcellular fractionation may help enrich the target for detection.
• Cross-reactivity with human, mouse, and rat has been reported; however, epitope conservation should be verified across species.
• The antibody is polyclonal; lot-to-lot consistency should be confirmed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For TGFB1I1-related cell adhesion, signaling, and nuclear receptor coactivation studies, CamelBio can source rare-target raw materials and support your project from concept to clinic with reliable supply solutions.