Anti-TGFA Rabbit Polyclonal Antibody for WB, ELISA - P01135

Core Product Specifications and Parameters

Biological Background: TGFA Function and Localization

• TGFA encodes protransforming growth factor alpha, a mitogenic polypeptide that binds to the EGF receptor (EGFR) and synergizes with TGF beta to promote anchorage-independent cell proliferation.
• The protein is synthesized as a transmembrane precursor and cleaved to release the mature growth factor; it localizes to the cell membrane and the extracellular space.
• Isoforms 1, 3, and 4 are expressed in keratinocytes and tumor-derived cell lines, contributing to autocrine and paracrine signaling.
• The mature growth factor contains an EGF-like domain and is modified by glycosylation and palmitoylation, as annotated by UniProt keywords.
• TGFA activates downstream pathways such as RAS/MAPK, driving cell proliferation, differentiation, and development.
• Alternative splicing generates multiple isoforms, and aberrant TGFA expression is associated with cancer progression.

Experimental Guidance and Technical Tips

• The immunogen covers the mature TGFA sequence (amino acids 24-98), suggesting the antibody likely recognizes the soluble growth factor form.
• For WB, consider using reduced and non-reduced conditions due to potential disulfide bonds in the EGF-like domain.
• Cross-reactivity with mouse indicates conserved epitopes; validate detection in the relevant cell or tissue lysates.
• In ELISA, include appropriate controls such as recombinant TGFA protein or blocking peptides to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined one-stop sourcing solution for IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-TGFA product. Our platform simplifies procurement of bulk ancillary reagents and supports the development of assays targeting the EGFR signaling pathway. Whether you require rare target antibodies or complete raw-material packages, CamelBio bridges the gap from concept to clinic.