Anti-TGF beta Receptor I (TGFBR1) Rabbit Monoclonal Antibody for WB and ELISA - P36897

Core Product Specifications and Parameters

Biological Background: TGF-beta receptor type-1 (TGFBR1) Function and Localization

• TGFBR1 encodes a single-pass transmembrane serine/threonine kinase receptor that partners with TGFBR2 to form the non-promiscuous receptor for TGF-beta cytokines (TGFB1, TGFB2, TGFB3).
• Upon ligand binding, TGFBR2 phosphorylates and activates TGFBR1, which then phosphorylates SMAD2; the SMAD2-SMAD4 complex translocates to the nucleus to regulate transcription of target genes, defining the canonical TGF-beta pathway.
• TGFBR1 signaling governs a multitude of processes including cell cycle arrest, mesenchymal cell proliferation/differentiation, wound healing, extracellular matrix production, immunosuppression, and carcinogenesis. Related references: PMID:33914044
• In addition to SMAD-dependent signaling, TGFBR1 triggers non-canonical pathways such as TRAF6-mediated apoptosis and PARD6A phosphorylation-driven epithelial-mesenchymal transition.
• The receptor is predominantly located on the cell membrane, and also found at tight junctions, cell surface, and membrane rafts.
• TGFBR1 is ubiquitously expressed, with highest levels in placenta and lowest in brain and heart; it is also detected in numerous cancer cell lines. Related references: PMID:25893292
• UniProt keywords include kinase, receptor, apoptosis, disease variant, and glycoprotein, highlighting its signal transduction and disease relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of human TGFBR1, which may ensure recognition of both native and denatured protein conformations, making it well-suited for Western blot under reducing/denaturing conditions.
• For Western blot, expect a specific band at approximately 56 kDa; include positive and negative cell/tissue lysates to confirm specificity, and consider optimizing antibody dilution and incubation conditions for your sample type.
• This antibody is also validated for ELISA; for sandwich ELISA applications, assess its performance as either capture or detection antibody in combination with a suitable pair, and titrate coating concentrations.
• Always include appropriate controls (e.g., untreated vs. ligand-stimulated samples) to monitor TGFBR1 phosphorylation status if probing signaling events.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined pathway to high-quality IVD raw materials for TGF-beta signaling and cancer research. From this TGFBR1 monoclonal antibody to matched antibody pairs, bulk reagents, and custom sourcing for rare targets, we support your entire development workflow. Partner with CamelBio to accelerate your assay development with reliable, validated reagents and expert technical consultation.