Anti-TGF beta 1 Polyclonal Antibody for WB, IF/ICC, ELISA - P01137

Core Product Specifications and Parameters

Biological Background: TGF beta 1 Function and Localization

• TGFB1 encodes a proprotein cleaved into Latency-associated peptide (LAP) and the active TGF-beta-1 cytokine; LAP maintains TGF-beta-1 in a latent state during extracellular matrix storage and regulates its activation. Related references: PMID:28117447 PMID:29109152
• TGF-beta-1 is a multifunctional growth factor involved in cell proliferation, differentiation, immune regulation, and tissue homeostasis. It signals via TGFBR1/TGFBR2 receptors, activating SMAD2/3-mediated transcription. Related references: PMID:20207738
• Control of TGF-beta-1 latency involves interactions with milieu molecules such as LTBP1, LRRC32/GARP (on Tregs), and LRRC33/NRROS (in macrophages/microglia). Integrins ITGAV:ITGB6 or ITGAV:ITGB8 bind LAP, triggering a conformational change that releases active TGF-beta-1. Related references: PMID:19651619 PMID:19750484 PMID:2022183 PMID:22278742 PMID:28117447
• TGF-beta-1 stimulates sustained collagen production in osteoblasts through regulated intramembrane proteolysis of CREB3L1 and promotes bone formation by chemotaxis and differentiation of osteoblasts. Related references: PMID:25310401
• TGF-beta-1 induces epithelial-to-mesenchymal transition (EMT) and cell migration, processes critical for development and cancer metastasis, via SMAD2/3 phosphorylation. Related references: PMID:25893292 PMID:30696809
• Highly expressed in bone tissue, articular cartilage, and chondrocytes; expression levels are elevated in osteoarthritis lesions, where it colocalizes with ASPN. Related references: PMID:11746498 PMID:17827158
• Post-translational modifications include cleavage on basic residues, N-linked glycosylation, and formation of multiple disulfide bonds, essential for correct folding and latency.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 291–390 within the mature TGF-beta-1 domain. For WB, consider that under reducing conditions the antibody may detect the cleaved mature monomer (~12.5 kDa), while non-reducing conditions may reveal the 44 kDa proprotein or disulfide-linked dimers. Validate band patterns in your sample system.
• For IF/ICC, employ permeabilization to detect intracellular TGF-beta-1 in Golgi/secretory vesicles; for secreted targets, consider Brefeldin A treatment to block secretion.
• In ELISA, the antibody can be used for capture/detection of TGF-beta-1. If measuring total TGF-beta-1 in biological fluids, pre-treatment to activate latent complexes (e.g., acid activation) may be necessary.
• Cross-reactivity with mouse and rat TGF-beta-1 has been reported; verify performance if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing—all designed to accelerate your assay development from concept to clinic. This anti-TGF beta 1 polyclonal antibody addresses critical needs in fibrosis, immuno-oncology, and regenerative medicine research, and CamelBio can further support your projects with tailored raw-material solutions and technical consulting.