Anti-TGF-beta-1 Polyclonal Antibody for WB, ELISA - P04202

Core Product Specifications and Parameters

Biological Background: TGF-beta-1 Function and Localization

• Synthesized as a proprotein that is cleaved into Latency-associated peptide (LAP) and active TGF-beta-1; the two chains remain non-covalently associated to keep TGF-beta-1 latent in the extracellular matrix. Related references: PMID:29909984
• Activation requires release from LAP via integrins (ITGAV:ITGB6 or ITGAV:ITGB8) that distort LAP; this process is tightly regulated by milieu molecules like LTBP1, LRRC32/GARP, and LRRC33/NRROS. Related references: PMID:10025398 PMID:29909984
• Multifunctional cytokine regulating cell growth, differentiation, immune function, microglia responses, and neurodegeneration. Related references: PMID:22781750 PMID:29909984
• Promotes bone remodeling by stimulating osteoblast chemotaxis, proliferation, and differentiation. Related references: PMID:22781750
• Modulates T-cell lineage differentiation: high TGF-beta-1 concentrations favor regulatory T-cell (Treg) development, while low concentrations in concert with IL-6 and IL-21 promote Th17 differentiation. Related references: PMID:18368049
• Positively regulates odontoblastic differentiation in dental papilla cells via SMAD2/3 phosphorylation and IPO7-mediated nuclear translocation. Related references: PMID:33548622
• Induces epithelial-to-mesenchymal transition (EMT) and cell migration.
• Secreted and deposited in the extracellular matrix.
• Expressed in cardiomyocytes and weakly in mammary glands, with increased expression during involution.

Experimental Guidance and Technical Tips

• The immunogen (aa 30–390) covers the LAP region and the active TGF-beta-1 domain, potentially detecting both the proprotein (~44 kDa) and the mature TGF-beta-1 chain (~12.5 kDa) in Western blot; consider running appropriate controls.
• For Western blot, validate the antibody in mouse or rat tissue lysates known to express TGF-beta-1 (e.g., spleen, lung, bone).
• For ELISA applications, the antibody may be suitable as a capture or detection reagent; confirm specificity with recombinant TGF-beta-1 standards.
• Optimize antibody dilution and buffer conditions experimentally for your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This anti‑TGF‑beta‑1 antibody supports research in immunology, cell differentiation, and disease models. For your sourcing needs, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist with the procurement of rare‑target raw materials.