Anti-TGF beta 1 Monoclonal Antibody for WB, IHC, IF, ELISA - P01137

Core Product Specifications and Parameters

Biological Background: TGF beta 1 Function and Localization

• The TGF beta 1 gene encodes a proprotein that is cleaved into the Latency-associated peptide (LAP) and the active Transforming growth factor beta-1 (TGF-beta-1) chain, which together regulate the latent storage and activation of this multifunctional cytokine.
• TGF-beta-1 is maintained in an inactive state in the extracellular matrix through non-covalent association with LAP, and its activation is controlled by interactions with ‘milieu molecules’ such as LTBP1, LRRC32/GARP, and LRRC33/NRROS (PubMed:19651619, PubMed:19750484, PubMed:2022183). Related references: PMID:19651619, PMID:19750484, PMID:2022183
• Integrin-mediated distortion of the LAP chain (by ITGAV:ITGB6 or ITGAV:ITGB8) results in release of active TGF-beta-1, which then binds to its receptors TGFBR1 and TGFBR2 to initiate SMAD2/3 phosphorylation and downstream signaling (PubMed:22278742, PubMed:28117447, PubMed:25893292). Related references: PMID:22278742, PMID:28117447, PMID:25893292
• TGF-beta-1 plays a critical role in immune regulation, promoting either T-helper 17 (Th17) or regulatory T-cell (Treg) lineage differentiation in a concentration-dependent manner (By similarity).
• It is a potent regulator of bone remodeling, stimulating osteoblastic bone formation, chemotaxis, proliferation, and differentiation (By similarity), and is highly expressed in bone and articular cartilage, with elevated levels in osteoarthritis (PubMed:11746498, PubMed:17827158). Related references: PMID:11746498, PMID:17827158
• TGF-beta-1 induces epithelial-to-mesenchymal transition (EMT) and cell migration, and stimulates sustained collagen production via regulated intramembrane proteolysis of CREB3L1 (PubMed:25310401, PubMed:30696809). Related references: PMID:25310401, PMID:30696809
• The protein is secreted into the extracellular space and matrix, and post-translational modifications include disulfide bond formation and glycosylation (as indicated by UniProtKB keywords).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering part of the TGF beta 1 sequence; consider verifying the antibody’s epitope specificity in your target species.
• For Western blot, a band around 44 kDa (proprotein) or 12.5 kDa (mature form after processing) may be detected depending on the sample; validate using appropriate positive controls (e.g., TGF-beta-treated cells).
• In immunohistochemistry and immunofluorescence, antigen retrieval and permeabilization optimization are advised for intracellular or extracellular matrix detection.
• For ELISA applications, pairing with a suitable capture antibody may be required; confirm the antibody’s reactivity with the analyte of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary bulk reagents, to support diagnostic assay development. This TGF beta 1 antibody is a reliable tool for researchers investigating immune regulation, bone remodeling, and fibrosis pathways. With a global sourcing network, CamelBio can also swiftly procure rare-target raw materials to accelerate your project from concept to clinic.