Anti-TFR2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UP52

Core Product Specifications and Parameters

Biological Background: TFR2 (Transferrin Receptor Protein 2) Function and Localization

• TFR2 (Transferrin receptor protein 2), also known as HFE3 or TFRC2, mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner.
• It plays a key role in iron metabolism, hepatocyte function, and erythrocyte differentiation.
• The receptor exists in multiple isoforms due to alternative splicing; the alpha form is predominantly hepatic, while a beta form is weakly expressed across many tissues.
• Subcellularly, TFR2 localizes to the cell membrane and cytoplasm.
• It is a glycoprotein with disulfide bonds and a single-pass transmembrane helix, featuring a signal-anchor topology.
• Mutations in TFR2 are associated with hereditary hemochromatosis, an iron overload disorder.
• Protein expression is highest in liver, with lower levels in spleen, lung, muscle, prostate, and peripheral blood mononuclear cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 130–330, a region not encompassing the full ectodomain; consider this when designing competitive or sandwich assays.
• For Western blot, TFR2 runs at approximately 89–90 kDa; use reducing conditions and validate with a known positive control such as HepG2 liver cell lysates.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; liver sections can serve as a suitable positive control due to high expression.
• The antibody cross-reacts with mouse TFR2, making it useful for mouse model studies; however, verify reactivity in your specific mouse strain.
• As a polyclonal antibody, lot-to-lot consistency may vary; include appropriate positive and negative controls in every experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this anti-TFR2 polyclonal antibody for iron metabolism and hepatocyte studies. Our one-stop platform offers validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials—backed by technical and sourcing expertise to support your workflow from discovery to clinical application.