Anti-TFPI Rabbit Polyclonal Antibody for WB, ELISA - P10646

Core Product Specifications and Parameters

Biological Background: TFPI Function and Localization

• TFPI (Tissue factor pathway inhibitor), also known as Extrinsic pathway inhibitor (EPI) or Lipoprotein-associated coagulation inhibitor (LACI), is a Kunitz-type serine protease inhibitor that centrally regulates the extrinsic coagulation pathway.
• It directly inhibits factor Xa and, in a factor Xa-dependent manner, inhibits the tissue factor/factor VIIa complex, thereby suppressing thrombin generation and exerting antithrombotic effects.
• TFPI is primarily synthesized in endothelial cells, consistent with its role in vascular hemostasis, and is secreted into the bloodstream.
• The protein associates with lipoproteins in plasma and is also found anchored to the microsome membrane via a GPI anchor, indicating both soluble and membrane-bound forms.
• Post-translational modifications include N-glycosylation, formation of disulfide bonds, and a C-terminal GPI-anchor attachment, which are critical for its stability and localization.
• Alternative splicing of the TFPI gene yields multiple isoforms, which may differ in their C-terminal regions and affinity for lipoproteins and cell surfaces.
• Given its inhibitory role, TFPI is a key anticoagulant factor; deficiencies or dysregulation have been implicated in thrombotic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-224 of human TFPI, covering the Kunitz domain 1; this suggests suitability for detecting TFPI isoforms containing this region in human, mouse, and rat samples.
• For Western blot, the predicted band is around 35 kDa; however, glycosylation and potential GPI-anchor removal may cause multiple bands or smears in cell lysates or plasma samples.
• To reduce non-specific binding typical of polyclonal antibodies, consider optimizing blocking and wash conditions when using complex samples like serum.
• For ELISA applications, TFPI is secreted, so plasma or culture supernatants may be appropriate sample types.
• Validate antibody performance under your specific experimental conditions, as cross-reactivity with mouse and rat TFPI has been reported but requires verification in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless supply chain for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for hemostasis and thrombosis research. Our TFPI Rabbit pAb supports diagnostic development and coagulation studies, backed by one-stop sourcing capabilities. Contact us for customized solutions and technical consultation to accelerate your IVD product development.