Anti-TFPI Rabbit Monoclonal Antibody for WB, ELISA - P10646

Core Product Specifications and Parameters

Biological Background: TFPI Function and Localization

• Tissue factor pathway inhibitor (TFPI), also known as extrinsic pathway inhibitor (EPI) or lipoprotein-associated coagulation inhibitor (LACI), is a key regulator of the coagulation cascade.
• It directly inhibits factor Xa and, in a factor Xa-dependent manner, inhibits the tissue factor/factor VIIa (TF/VIIa) complex, likely by forming a quaternary Xa/TFPI/VIIa/TF complex.
• TFPI exhibits antithrombotic properties and can associate with plasma lipoproteins.
• The protein is predominantly secreted and also localizes to the microsome membrane.
• It is mostly expressed in endothelial cells, reflecting its crucial role in vascular hemostasis.
• TFPI belongs to the serine protease inhibitor family and contains characteristic disulfide bonds and a Kunitz-type domain repeat.
• Alternative splicing generates multiple isoforms, which may differ in their association with lipoproteins or glycosylphosphatidylinositol (GPI)-anchor attachment.
• Post-translational modifications include glycosylation and a potential GPI-anchor, contributing to its membrane association.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 200-304 of human TFPI, a region that may provide specificity for the Kunitz-2 domain, consider this when designing competition or epitope mapping studies.
• For Western blot applications, a band around 35 kDa is expected; validate in relevant human endothelial or plasma samples where TFPI is endogenously expressed.
• In ELISA, pair this antibody with a suitable capture/detection antibody while verifying against purified TFPI or known plasma controls to ensure proper assay performance.
• This rabbit monoclonal antibody offers consistent affinity and reduced lot-to-lot variation; confirm its reactivity under your specific experimental conditions and sample types.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material sourcing partner, CamelBio provides reliable access to this high-quality TFPI antibody and the full suite of coagulation-related reagents. Whether you need validated monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw materials for diagnostic kit development, our B2B platform streamlines your procurement from concept to clinic.