Anti-TFF2 Polyclonal Antibody for WB, ELISA - Q03403

Core Product Specifications and Parameters

Biological Background: TFF2 Function and Localization

• TFF2 (Trefoil factor 2), also referred to as Spasmolysin or Spasmolytic polypeptide (SP), is encoded by the TFF2 gene.
• It functions as an inhibitor of gastrointestinal motility and gastric acid secretion, and may serve as a structural component of gastric mucus by stabilizing glycoproteins via carbohydrate interactions.
• TFF2 is a secreted protein predominantly expressed in the stomach.
• The protein contains multiple disulfide bonds and a signal peptide, typical of secreted trefoil factors.
• UniProt keywords include Disulfide bond, Repeat, Secreted, and Signal, reflecting its structural and secretory nature.
• The observed molecular weight is approximately 14 kDa, consistent with the calculated mass of 14284 Da.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24-129 of the mature human TFF2 protein, covering the trefoil domain; this region is likely to generate antibodies recognizing both native and denatured forms.
• For Western blotting, consider testing a range of antibody dilutions (e.g., 1:500–1:2000) on gastric tissue lysates or recombinant TFF2 to optimize signal specificity.
• In ELISA applications, use the antibody as a capture or detection reagent, and validate performance using recombinant TFF2 standards.
• As TFF2 is a secreted protein with potential glycosylation, ensure sample preparation is compatible with detection (e.g., deglycosylation enzymes may be needed if glycan-dependent epitopes are masked).
• Cross-reactivity is validated for human; if working with other species, check sequence homology and perform pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by providing a comprehensive range of IVD raw materials, including this anti-TFF2 polyclonal antibody for gastrointestinal function studies. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your assay development from concept to clinic.