Anti-TFF1/pS2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04155

Core Product Specifications and Parameters

Biological Background: TFF1/pS2 Function and Localization

• TFF1 (Trefoil factor 1) is also known as breast cancer estrogen-inducible protein (pS2), PNR-2, polypeptide P1.A (hP1.A), commonly referred to as pS2, BCEI, HPS2, HP1.A, pNR-2, and D21S21.
• It functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa, providing a physical barrier against noxious agents, and may inhibit the growth of calcium oxalate crystals in urine.
• The protein is secreted, containing a signal peptide and forming disulfide bonds characteristic of the trefoil factor family.
• TFF1 is strongly expressed in normal gastric mucosa and in regenerative tissues surrounding ulcerous lesions of the gastrointestinal tract, but lower expression is found in gastric cancer.
• It is detected in goblet cells of the intestines, submucosal glands of the esophagus, trachea, and vagina, as well as in urine; weakly in bronchial goblet cells.
• TFF1 is strongly expressed in breast cancer while normal mammary tissue shows low levels; its expression is regulated by estrogen in MCF-7 cells.
• According to UniProt keywords, it is a growth factor, has a 3D-structure determined, and is identified in proteomics studies.

Experimental Guidance and Technical Tips

• For WB, given the small protein weight of TFF1 (~9 kDa), consider using a 15% SDS-PAGE gel and optimize transfer to low-molecular-weight PVDF or nitrocellulose membranes to ensure retention.
• When performing IHC-P, validate in known positive tissues such as human gastric mucosa or breast carcinoma, and include appropriate antigen retrieval steps.
• For ELISA experiments, this polyclonal antibody can be considered as a detection or capture reagent after proper titration and pairing with a matched antibody.
• Since the immunogen corresponds to amino acids 25-84, verify reactivity against your target sequence in the relevant species and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing reliable access to optimized antibodies like anti-TFF1/pS2 for gastrointestinal and cancer research. Beyond single reagents, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your diagnostic development from concept to clinic.