Anti-TFEB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P19484

Core Product Specifications and Parameters

Biological Background: TFEB Function and Localization

• Transcription factor EB (TFEB), a member of the MiT/TFE family (also known as bHLHe35), serves as a master regulator of lysosomal biogenesis, autophagy, and energy metabolism (PubMed:21617040, PubMed:22343943, PubMed:22576015). Related references: PMID:21617040 PMID:22343943 PMID:22576015
• Nutrient availability controls TFEB activity: MTOR-dependent phosphorylation promotes cytosolic retention; starvation-induced dephosphorylation triggers nuclear import and transcriptional activation of lysosomal and autophagy genes (PubMed:22343943, PubMed:22576015, PubMed:22692423). Related references: PMID:22343943 PMID:22576015 PMID:22692423
• Dimerization with other MiT/TFE family members like TFE3 or MITF is required for efficient DNA binding to E-box and CLEAR-box promoter sequences (PubMed:1748288, PubMed:19556463, PubMed:29146937). Related references: PMID:1748288 PMID:19556463 PMID:29146937
• TFEB regulates lysosomal positioning by inducing PIP4P1 expression and drives autophagy upon glucose deprivation in cooperation with ACSS2 (PubMed:29146937, PubMed:28552616). Related references: PMID:29146937 PMID:28552616
• In adaptive immunity, TFEB activates CD40L expression in T-cells, contributing to thymus-dependent antibody responses (By similarity).
• The protein localizes to the nucleus, cytoplasm, and lysosome membrane, with its shuttling dynamically controlled by nutrient and stress signals.
• During bacterial infection, itaconate-mediated alkylation of TFEB induces nuclear translocation and lysosomal biogenesis, facilitating bacterial clearance (PubMed:35662396). Related references: PMID:35662396
• TFEB also plays roles in placental vascularization (By similarity) and is subject to multiple post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 391-461) of human TFEB, which may recognize full-length TFEB and possibly post-translational modification variants; validate in the relevant sample system to confirm specificity.
• For Western blot, a band around 52 kDa is expected; consider using nuclear/cytoplasmic fractionation protocols to monitor TFEB translocation in response to stimuli.
• In IHC-P, antigen retrieval and primary antibody dilution should be optimized; include positive control tissues known to express TFEB (e.g., kidney, liver) and appropriate negative controls.
• For IF/ICC, ensure that fixation and permeabilization conditions preserve nuclear morphology; co-staining with lysosomal markers can assess TFEB activation status.
• When using ELISA, titrate the antibody carefully and include relevant blocking and washing steps to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials and technical services, from concept to clinic. This anti-TFEB antibody supports research in autophagy, lysosomal biology, and transcriptional regulation. Beyond high-quality antibodies, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials to streamline your assay development and production needs.