Anti-TFEB Polyclonal Antibody for WB, IP, ELISA - Q9R210

Core Product Specifications and Parameters

Biological Background: TFEB Function and Localization

• Transcription factor EB (TFEB), also known as Tcfeb and bHLHe35, is a key member of the MiT/TFE family of transcription factors and functions as a master regulator of lysosomal biogenesis, autophagy, and energy metabolism. Related references: PMID:16936731, PMID:22343943, PMID:27278822
• TFEB specifically recognizes E-box sequences (5'-CANNTG-'), and efficient DNA-binding requires dimerization with itself or other MiT/TFE family members (e.g., TFE3, MITF). It also binds the CLEAR-box sequence (5'-GTCACGTGAC-3') in promoters of lysosomal genes. Related references: PMID:16936731, PMID:27278822
• TFEB activity is tightly regulated by MTOR-dependent phosphorylation: in nutrient-rich conditions, phosphorylation retains TFEB in the cytoplasm; upon starvation or lysosomal stress, MTOR inhibition leads to dephosphorylation, nuclear translocation, and transcriptional activation of target genes. Related references: PMID:35662396
• TFEB promotes autophagy by upregulating gene expression involved in autophagic pathways, and also regulates lysosomal positioning in response to nutrient deprivation by inducing PIP4P1 expression. Related references: PMID:27278822, PMID:29146937
• TFEB plays a dual role in immunity: it activates CD40L in T-cells to support antibody responses and orchestrates antibacterial innate immunity through itaconate-mediated alkylation, which drives lysosomal biogenesis for pathogen clearance. Related references: PMID:16936731, PMID:24882217, PMID:27184844, PMID:35662396
• TFEB is widely expressed and dynamically shuttles between the nucleus, cytoplasm, and lysosome membrane, enabling rapid responses to metabolic and immune signals.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390–476 in the C-terminal region of mouse TFEB, a domain that may detect both full-length and truncated forms of the protein.
• For Western blot, consider using whole-cell lysates or nuclear extracts from human or mouse cells; nutrient-starvation or Torin 1 treatment can enhance nuclear TFEB signal and may improve detection sensitivity.
• In immunoprecipitation, optimal antibody concentration should be titrated for the target species and sample type; inclusion of appropriate positive and negative controls is recommended.
• For ELISA, validate performance using recombinant TFEB protein or characterized cell lysates to ensure specificity and linear range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing and supply, delivering validated antibodies, recombinant proteins, and bulk ancillary reagents. With dedicated expertise in autophagy, lysosomal biology, and transcriptional regulation, CamelBio supports diagnostic manufacturers and laboratories in developing robust assays for challenging targets like TFEB. From concept to clinic, we streamline procurement and provide tailored technical consultation to accelerate your project’s success.