Anti-TFCP2 Rabbit Polyclonal Antibody for WB, ELISA - Q12800

Core Product Specifications and Parameters

Biological Background: TFCP2 Function and Localization

• TFCP2, also known as alpha-globin transcription factor CP2, LSF, or SEF, is a transcription factor encoded by the TFCP2 gene in humans. It belongs to the CP2 transcription factor family and is involved in the regulation of multiple cellular and viral promoters.
• The protein binds to the alpha-globin promoter in erythroid cells and activates its transcription through synergistic interaction with UBP1 (by similarity).
• TFCP2 is a component of the stage selector protein (SSP) complex, facilitating the interaction of gamma-globin genes with enhancer elements in the locus control region during fetal erythropoiesis.
• It also recognizes and binds to promoters of fibrinogen, SV40, and HIV-1, indicating a broad role in transcriptional regulation.
• Subcellularly, TFCP2 localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcription factor.
• The protein undergoes phosphorylation and is subject to alternative splicing, generating multiple isoforms. It is ubiquitously expressed across tissues, with detected protein in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart, and lung.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-280 of human TFCP2 (NP_005644.2). This N-terminal region is conserved in mouse (as indicated by cross-reactivity), ensuring reliable detection across species.
• For Western blotting (WB), consider using nuclear extracts from human or mouse cell lines (e.g., HeLa, K562, or NIH/3T3) to enrich for TFCP2, which primarily localizes to the nucleus.
• In ELISA applications, the antibody can be used to capture or detect recombinant TFCP2 protein fragments. Validate the assay with appropriate controls and optimize coating/blocking conditions.
• Given TFCP2’s role in hematopoiesis, consider testing in erythroid differentiation models (e.g., induced K562 or CD34+ cells) to confirm target engagement.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-TFCP2 polyclonal antibody supports chromatin and transcription factor research, with CamelBio providing scalable supply, customized bulk orders, and technical consultation from concept to clinic. Rely on CamelBio’s integrated platform to streamline your diagnostic development pipeline.